When should I use the standard vs. the fast protocol for the EZ2 RNA FFPE Kit?

The FFPE RNA fast protocol provides a streamlined and time efficient workflow for the extraction of RNA to be used in downstream applications like NGS. If longer decrosslinking is required, the standard protocol offers an extended crosslink removal step. Note that incubation at elevated temperature may impact RNA integrity. This longer protocol results in a higher yield, which is beneficial for PCR reactions but might be less suited for NGS.