What is the size, charge and isoelectric point of the DHFR protein in the pQE vectors?
The native Dihydrofolate Reductase (DHFR) protein has the following technical features:
- Molecular Weight: 21.5 kDa
- Isoelectric Point: 8.5
- Charge at pH 7.0: 2.2
DHFR expressed in QIAexpress vectors pQE-16 and pOE-40 has the following technical features:
pQE-16
- Molecular Weight: 23.1 kDa
- Isoelectric Point: 9.1
- Charge at pH 7.0: 5.2
pQE-40
- Molecular Weight: 24.5 kDa
- Isoelectric Point: 9.2
- Charge at pH 7.0: 7.2