Is it advisable to include the IC in the extraction procedure?
The majority of kits provide the IC in a separate, green-colored capped tube for the benefit of the customer. The user can include the IC in the extraction procedure, thereby controlling the efficiency of the DNA/RNA preparation and checking for potential PCR inhibition in the subsequent PCR.
Alternatively, the IC may be used for PCR inhibition control only by adding it to the artus® Master directly. The user manual provides corresponding pipetting schemes for both variants