Transfection Cell Database - Detailed View


Cell Line: HeLa

Cell Line Species/Tissue: Human /  
Transfection Reagent: Attractene 
Nucleic Acid:
DNA 
Growth Medium:
MEM +FBS, L-glutamine, sodium pyruvate 
Percent Serum (%):
10 
Reporter System:
Western Blot anti-FLAG 
Plasmid Purification Method:
Endofree Plasmid Kit 
Plate Format:
6-well plate 
Number of Cells:
5E5/well 
Percent Confluence(%):
 
Amount of Nucleic Acid (µg):
Amount of Enhancer (µl):
 
Amount of Reagent (µl):
2.25 
Complex Incubation on Cells (hrs):
24 
Analysis Performed Post-Transfection (hrs):
Transfection Efficiency (%):
n/a 
 
Any modifications to the protocol?:
No modifications.  
Notes:
 
References: