| Cell Line Species/Tissue: |
Human
/
Embryo kidney |
| Transfection Reagent: |
TransMessenger |
Nucleic Acid:
|
siRNA (dsRNA) |
Growth Medium:
|
DMEM +10% FBS + Pen/Strep |
Percent Serum (%):
|
No serum |
Reporter System:
|
|
Plasmid Purification Method:
|
|
Plate Format:
|
24-well |
Number of Cells:
|
5x10e5/well |
Percent Confluence(%):
|
~60% |
Amount of Nucleic Acid (µg):
|
0.8 µg |
Amount of Enhancer (µl):
|
1.6 µg |
Amount of Reagent (µl):
|
4 µl |
Complex Incubation on Cells (hrs):
|
3 h |
Analysis Performed Post-Transfection (hrs):
|
24-48 h |
Transfection Efficiency (%):
|
~90% |
| Knockdown Efficiency (%): |
|
| |
| Any modifications to the protocol?:
|
| none |
| Notes: |
| Compared TKO, Oligofectamine, and TransMessenger in parallel experiments.
TKO: 30%
Oligofectamine: 50%
TransMessenger: 90% |
| References: |
| |