Transfection Cell Database - Detailed View


Cell Line: SH-SY5Y

Cell Line Species/Tissue: human / neuroblastoma brain 
Transfection Reagent: Effectene 
Nucleic Acid:
DNA 
Growth Medium:
RPMI1640 +Glutamax +Penstrep +Serum 
Percent Serum (%):
10 
Reporter System:
GFP 
Plasmid Purification Method:
 
Plate Format:
60-mm dish 
Number of Cells:
 
Percent Confluence(%):
40 
Amount of Nucleic Acid (µg):
Amount of Enhancer (µl):
Amount of Reagent (µl):
25 
Complex Incubation on Cells (hrs):
20 
Analysis Performed Post-Transfection (hrs):
48 
Transfection Efficiency (%):
20-25 
 
Any modifications to the protocol?:
 
Notes:
 
References: