| Cell Line Species/Tissue: |
Human
/
Cervix; epithelial; adenocarcinoma |
| Transfection Reagent: |
HiPerFect |
Nucleic Acid:
|
siRNA (dsRNA) |
Growth Medium:
|
DMEM/F12 |
Percent Serum (%):
|
10 % |
Reporter System:
|
|
Plasmid Purification Method:
|
|
Plate Format:
|
60 mm |
Number of Cells:
|
6x10e5 |
Percent Confluence(%):
|
90 % |
Amount of Nucleic Acid (µg):
|
1nM,10nM,5nM |
Amount of Enhancer (µl):
|
|
Amount of Reagent (µl):
|
5 µl |
Complex Incubation on Cells (hrs):
|
24 h, 48 h |
Analysis Performed Post-Transfection (hrs):
|
24 h, 48 h |
Transfection Efficiency (%):
|
60~70% |
| Knockdown Efficiency (%): |
83.40 % |
| |
| Any modifications to the protocol?:
|
| Yes, see notes |
| Notes: |
| Amount of HiPerFect Reagent :20µl?5µl (in 60mm Dish)
Because 20µl of transfection reagent made quite many of cells died, but when I used 5µl transfection reagent, almost cells was not dead and transfection has been successful.
Analysis method: real-time PCR |
| References: |
| |