| Cell Line Species/Tissue: |
human
/
neuroblastoma |
| Transfection Reagent: |
Effectene |
Nucleic Acid:
|
DNA |
Growth Medium:
|
DMEM/F12 (1:1) |
Percent Serum (%):
|
5% |
Reporter System:
|
Luciferase, B-galactosidase |
Plasmid Purification Method:
|
|
Plate Format:
|
6-well plate |
Number of Cells:
|
0.3 * 10e6 cells/well |
Percent Confluence(%):
|
50-70% |
Amount of Nucleic Acid (µg):
|
0.4µg |
Amount of Enhancer (µl):
|
3.2µl |
Amount of Reagent (µl):
|
4µl |
Complex Incubation on Cells (hrs):
|
18h |
Analysis Performed Post-Transfection (hrs):
|
48 |
Transfection Efficiency (%):
|
20% |
| |
| Any modifications to the protocol?:
|
| reduced concentration of effectene |
| Notes: |
| Customer compared Effectene to Calcium Phosphate.
Effectene Reagent: 20%
Calcium Phosphate: 5% |
| References: |
| |