| Cell Line Species/Tissue: |
mouse
/
B-cell lymphoma |
| Transfection Reagent: |
Effectene |
Nucleic Acid:
|
DNA |
Growth Medium:
|
IMDM, 5% FBS |
Percent Serum (%):
|
5 |
Reporter System:
|
Luciferase, pGL3-basic (Promega) |
Plasmid Purification Method:
|
QIAGEN Plasmid Kit |
Plate Format:
|
24-well plate |
Number of Cells:
|
500,000 |
Percent Confluence(%):
|
|
Amount of Nucleic Acid (µg):
|
0.6 |
Amount of Enhancer (µl):
|
4.8 |
Amount of Reagent (µl):
|
6 |
Complex Incubation on Cells (hrs):
|
24 |
Analysis Performed Post-Transfection (hrs):
|
24 |
Transfection Efficiency (%):
|
359,933 RLU |
| |
| Any modifications to the protocol?:
|
| |
| Notes: |
| Grow cells to 8 x 10e5 cells per ml, then split the cells the day prior to transfection. |
| References: |
| Goldstein, A. and Scheuermann, R.H. (1999) Reagent for transfection of the mature B-cell line M12.4. QIAGEN News 1999 No.1 |