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Transfection Cell Database - Detailed View


Cell Line: NIH3T3

Cell Line Species/Tissue: mouse / embryo fibroblast 
Transfection Reagent: Attractene 
Nucleic Acid:
DNA 
Growth Medium:
DMEM 
Percent Serum (%):
10% 
Reporter System:
ß-Galactosidase 
Plasmid Purification Method:
 
Plate Format:
96-well 
Number of Cells:
2x 10^4 (seeded one day before) 
Percent Confluence(%):
 
Amount of Nucleic Acid (µg):
0,2 µg 
Amount of Enhancer (µl):
 
Amount of Reagent (µl):
2 µl 
Complex Incubation on Cells (hrs):
 
Analysis Performed Post-Transfection (hrs):
48h 
Transfection Efficiency (%):
80U 
 
Any modifications to the protocol?:
Yes, final volume of diluted DNA was 30 µl instead of 50 µl  
Notes:
 
References: