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Transfection Cell Database - Detailed View


Cell Line: Primary NHBE

Cell Line Species/Tissue: Human / Primary bronchial epithelial 
Transfection Reagent: HiPerFect 
Nucleic Acid:
siRNA (dsRNA) 
Growth Medium:
BEGM 
Percent Serum (%):
0% 
Reporter System:
 
Plasmid Purification Method:
 
Plate Format:
24-well plate 
Number of Cells:
60,000 cells/well 
Percent Confluence(%):
~60-80% 
Amount of Nucleic Acid (µg):
10 nM 
Amount of Enhancer (µl):
 
Amount of Reagent (µl):
3 µl 
Complex Incubation on Cells (hrs):
 
Analysis Performed Post-Transfection (hrs):
 
Transfection Efficiency (%):
 
Knockdown Efficiency (%): >85%  
 
Any modifications to the protocol?:
 
Notes:
Low-Volume Protocol (transfection in reduced volume for three hours), qRT-PCR  
References: