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Transfection Cell Database - Detailed View
| Cell Line: S2 Schneider cells
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| Cell Line Species/Tissue: |
Drosophila
/
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| Transfection Reagent: |
Effectene |
Nucleic Acid:
|
DNA |
Growth Medium:
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Percent Serum (%):
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Reporter System:
|
pCoHygro selection vector cotransfected |
Plasmid Purification Method:
|
QIAfilter Plasmid Kit |
Plate Format:
|
25 cm2 flasks |
Number of Cells:
|
5 000 000 |
Percent Confluence(%):
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Amount of Nucleic Acid (µg):
|
1.8 µg |
Amount of Enhancer (µl):
|
16 µl |
Amount of Reagent (µl):
|
20 µl |
Complex Incubation on Cells (hrs):
|
24 hrs |
Analysis Performed Post-Transfection (hrs):
|
168 hrs |
Transfection Efficiency (%):
|
80-90 % |
| |
| Any modifications to the protocol?:
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| Notes: |
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| References: |
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