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Transfection Cell Database - Detailed View


Cell Line: HeLa

Cell Line Species/Tissue: Human / Cervix 
Transfection Reagent: Effectene 
Nucleic Acid:
DNA 
Growth Medium:
 
Percent Serum (%):
10% 
Reporter System:
CAT, GFP 
Plasmid Purification Method:
QIAGEN Plasmid Kit 
Plate Format:
6-well plate 
Number of Cells:
200000 
Percent Confluence(%):
 
Amount of Nucleic Acid (µg):
0.4 µg 
Amount of Enhancer (µl):
3.2 µl 
Amount of Reagent (µl):
10 µl 
Complex Incubation on Cells (hrs):
8 hrs 
Analysis Performed Post-Transfection (hrs):
48 hrs 
Transfection Efficiency (%):
55 % 
 
Any modifications to the protocol?:
 
Notes:
Compared Effectne to Calcium Phosphate. Transfection efficiency with Calcium Phosphate: 15% Transfection efficiency with Effectene: 55%  
References: