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Transfection Cell Database - Detailed View
| Cell Line: Primary Sertoli Cell Cultures
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| Cell Line Species/Tissue: |
Rat
/
Testes |
| Transfection Reagent: |
Effectene |
Nucleic Acid:
|
DNA |
Growth Medium:
|
F-12/Dulbecco’s modified Eagle’s medium |
Percent Serum (%):
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Reporter System:
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Plasmid Purification Method:
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Plate Format:
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12-well plates or cover glass |
Number of Cells:
|
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Percent Confluence(%):
|
6x10e5 |
Amount of Nucleic Acid (µg):
|
~0.3µg of plasmid DNA |
Amount of Enhancer (µl):
|
|
Amount of Reagent (µl):
|
2.4 µl |
Complex Incubation on Cells (hrs):
|
24 h |
Analysis Performed Post-Transfection (hrs):
|
|
Transfection Efficiency (%):
|
~15% |
| |
| Any modifications to the protocol?:
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| |
| Notes: |
| Primary Sertoli cells cultured in vitro were transfected with plasmids containing the full-length TGF-ß3 (pCIneo/TGF-ß3) with ~15% efficiency. About 24 h after transfection, both the steady-state mRNA and protein levels of TGF-ß3 increased significantly. |
| References: |
| Differential Interactions between Transforming Growth Factor-ß3/TR1, TAB1, and CD2AP Disrupt Blood-Testis
Barrier and Sertoli-Germ Cell Adhesion
Weiliang Xia, Dolores D. Mruk, Will M. Lee, and C. Yan Cheng
THE JOURNAL OF BIOLOGICAL CHEMISTRY VOL. 281, NO. 24, pp. 16799–16813, June 16, 2006
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