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Transfection Cell Database - Detailed View
| Cell Line: Rat hepatic myofibroblast cells
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| Cell Line Species/Tissue: |
Rat
/
Liver |
| Transfection Reagent: |
Effectene |
Nucleic Acid:
|
DNA |
Growth Medium:
|
DMEM, 400 µg/ml G418 |
Percent Serum (%):
|
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Reporter System:
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Plasmid Purification Method:
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Plate Format:
|
6-well |
Number of Cells:
|
1×104 |
Percent Confluence(%):
|
70 % |
Amount of Nucleic Acid (µg):
|
1 µg |
Amount of Enhancer (µl):
|
|
Amount of Reagent (µl):
|
no information |
Complex Incubation on Cells (hrs):
|
|
Analysis Performed Post-Transfection (hrs):
|
|
Transfection Efficiency (%):
|
|
| |
| Any modifications to the protocol?:
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| |
| Notes: |
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| References: |
| Inhibition on the production of collagen type I, III of activated hepatic stellate cells by antisense TIMP-1 recombinant plasmid
Wen-Bin Liu, Chang-Qing Yang, Wei Jiang, Yi-Qing Wang, Jing-Sheng Guo, Bo-Ming He, Ji-Yao Wang
World J Gastroenterol 2003;9(2):316-319 |
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