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Transfection Cell Database - Detailed View
| Cell Line: Primary monocyte
|
|
| Cell Line Species/Tissue: |
Human
/
monocyte |
| Transfection Reagent: |
HiPerFect |
Nucleic Acid:
|
siRNA (dsRNA) |
Growth Medium:
|
RPMI1640 |
Percent Serum (%):
|
10 % |
Reporter System:
|
|
Plasmid Purification Method:
|
|
Plate Format:
|
24-well |
Number of Cells:
|
1x10e5 |
Percent Confluence(%):
|
|
Amount of Nucleic Acid (µg):
|
37.5 ng |
Amount of Enhancer (µl):
|
|
Amount of Reagent (µl):
|
no information |
Complex Incubation on Cells (hrs):
|
|
Analysis Performed Post-Transfection (hrs):
|
24 h |
Transfection Efficiency (%):
|
|
| Knockdown Efficiency (%): |
50 % |
| |
| Any modifications to the protocol?:
|
| None |
| Notes: |
| Analysis method: Real-Time RT-PCR |
| References: |
| |
|