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Transfection Cell Database - Detailed View


Cell Line: HEK-293

Cell Line Species/Tissue: Human / kidney epithelial 
Transfection Reagent: PolyFect 
Nucleic Acid:
DNA 
Growth Medium:
 
Percent Serum (%):
 
Reporter System:
GFP 
Plasmid Purification Method:
QIAGEN Plasmid Kit 
Plate Format:
100-mm dish 
Number of Cells:
 
Percent Confluence(%):
70% 
Amount of Nucleic Acid (µg):
10µg 
Amount of Enhancer (µl):
 
Amount of Reagent (µl):
10µl 
Complex Incubation on Cells (hrs):
24h 
Analysis Performed Post-Transfection (hrs):
24h 
Transfection Efficiency (%):
80% 
 
Any modifications to the protocol?:
 
Notes:
Customer compared PolyFect to SuperFect. Both gave 80% transfection efficiency.  
References: