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Transfection Cell Database - Detailed View


Cell Line: BC-3

Cell Line Species/Tissue: Primary effusion leukemia /  
Transfection Reagent: HiPerFect 
Nucleic Acid:
siRNA (dsRNA) 
Growth Medium:
 
Percent Serum (%):
 
Reporter System:
 
Plasmid Purification Method:
 
Plate Format:
6-well dishes  
Number of Cells:
1x10e5 cell 
Percent Confluence(%):
see reference 
Amount of Nucleic Acid (µg):
see reference 
Amount of Enhancer (µl):
 
Amount of Reagent (µl):
see reference 
Complex Incubation on Cells (hrs):
48h 
Analysis Performed Post-Transfection (hrs):
 
Transfection Efficiency (%):
 
Knockdown Efficiency (%): 80%  
 
Any modifications to the protocol?:
 
Notes:
 
References:
Regulation of Microfilament Organization by Kaposi Sarcoma-associated Herpes Virus-cyclin·CDK6 Phosphorylation of Caldesmon Maria Emanuela Cuomo, Axel Knebel, Georgina Platt, Nick Morrice, Philip Cohen, and Sibylle Mittnacht J. Biol. Chem., Oct 2005; 280: 35844 - 35858.