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Transfection Cell Database - Detailed View


Cell Line: WI-38

Cell Line Species/Tissue: Human / Lung fibroblast 
Transfection Reagent: HiPerFect 
Nucleic Acid:
siRNA (dsRNA) 
Growth Medium:
MEM with L-Glu; supplemented with Vitamins(1/100) and non essential aminoacids (1/100) 
Percent Serum (%):
20 % 
Reporter System:
 
Plasmid Purification Method:
 
Plate Format:
30 mm wells (mostly in 6-well plates) 
Number of Cells:
225,000 per well 
Percent Confluence(%):
Approx. 60-70 % 
Amount of Nucleic Acid (µg):
2 µg per well 
Amount of Enhancer (µl):
 
Amount of Reagent (µl):
80 µl 
Complex Incubation on Cells (hrs):
24 h 
Analysis Performed Post-Transfection (hrs):
72 h 
Transfection Efficiency (%):
90 % 
Knockdown Efficiency (%): 80 %  
 
Any modifications to the protocol?:
Adjusted the amount of HiPerFect Reagent  
Notes:
 
References: