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Transfection Cell Database - Detailed View


Cell Line: RINmF5

Cell Line Species/Tissue: Human / fibroblast 
Transfection Reagent: TransMessenger 
Nucleic Acid:
siRNA (dsRNA) 
Growth Medium:
RPMI 
Percent Serum (%):
 
Reporter System:
 
Plasmid Purification Method:
 
Plate Format:
12-well plates 
Number of Cells:
8x10*4 
Percent Confluence(%):
 
Amount of Nucleic Acid (µg):
5 µl 
Amount of Enhancer (µl):
5 µl 
Amount of Reagent (µl):
5 µl 
Complex Incubation on Cells (hrs):
4 h 
Analysis Performed Post-Transfection (hrs):
12 h 
Transfection Efficiency (%):
60-70% 
Knockdown Efficiency (%):  
 
Any modifications to the protocol?:
 
Notes:
 
References:
Nitric Oxide 10 (2004)112 - 118 Analysis of cytokine-induced NO-dependent apoptosis using RNA interference or inhibition by 14o0W Neil Beeharry et al.