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Transfection Cell Database - Detailed View
Cell Line Species/Tissue: |
Human
/
Hepatocarcinoma |
Transfection Reagent: |
HiPerFect |
Nucleic Acid:
|
siRNA (dsRNA) |
Growth Medium:
|
RPMI |
Percent Serum (%):
|
10% FCS |
Reporter System:
|
|
Plasmid Purification Method:
|
|
Plate Format:
|
96-well plate |
Number of Cells:
|
10.000 cells/well |
Percent Confluence(%):
|
50-60% |
Amount of Nucleic Acid (µg):
|
10nM |
Amount of Enhancer (µl):
|
|
Amount of Reagent (µl):
|
1µl |
Complex Incubation on Cells (hrs):
|
48h |
Analysis Performed Post-Transfection (hrs):
|
48h |
Transfection Efficiency (%):
|
|
Knockdown Efficiency (%): |
85% |
|
Any modifications to the protocol?:
|
None |
Notes: |
RT-PCR/ Westernblot |
References: |
|
|