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Transfection Cell Database - Detailed View


Cell Line: HeLa S3

Cell Line Species/Tissue: Human / Cervix; epithelial; adenocarcinoma 
Transfection Reagent: HiPerFect 
Nucleic Acid:
siRNA (dsRNA) 
Growth Medium:
F12K 
Percent Serum (%):
10% FCS 
Reporter System:
 
Plasmid Purification Method:
 
Plate Format:
24-well plate 
Number of Cells:
60.000 cells/well 
Percent Confluence(%):
 
Amount of Nucleic Acid (µg):
0.1 nM 
Amount of Enhancer (µl):
 
Amount of Reagent (µl):
3µl 
Complex Incubation on Cells (hrs):
48h 
Analysis Performed Post-Transfection (hrs):
48h 
Transfection Efficiency (%):
 
Knockdown Efficiency (%): 80-90%  
 
Any modifications to the protocol?:
None  
Notes:
QIAGEN CDC2, ERK2, Alexa-488 and Alexa-546 labeled siRNA; Western blot; qRT-PCR Analysis;  
References: