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Transfection Cell Database - Detailed View


Cell Line: NIHpEJ (NIH/3T3)

Cell Line Species/Tissue: Mouse / Fibroblast 
Transfection Reagent: RNAiFect 
Nucleic Acid:
siRNA (dsRNA) 
Growth Medium:
DMEM + Glu + Pen/Strep 
Percent Serum (%):
10% 
Reporter System:
 
Plasmid Purification Method:
 
Plate Format:
6-well 
Number of Cells:
5x10e4 
Percent Confluence(%):
50% 
Amount of Nucleic Acid (µg):
2µg 
Amount of Enhancer (µl):
 
Amount of Reagent (µl):
6µl 
Complex Incubation on Cells (hrs):
24h 
Analysis Performed Post-Transfection (hrs):
48h 
Transfection Efficiency (%):
Not determined 
Knockdown Efficiency (%): Reduction of Protein in Western Blot  
 
Any modifications to the protocol?:
No  
Notes:
 
References: