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Transfection Cell Database - Detailed View


Cell Line: TRM-6 and TRM-6/PDX-1 cells

Cell Line Species/Tissue: Human / Pancreatic endocrine cells 
Transfection Reagent: TransMessenger 
Nucleic Acid:
siRNA (dsRNA) 
Growth Medium:
DMEM+10%FCS+5.5mM Glucose 
Percent Serum (%):
10%FCS 
Reporter System:
 
Plasmid Purification Method:
 
Plate Format:
2-well chamber glass slides 
Number of Cells:
 
Percent Confluence(%):
70-80 % 
Amount of Nucleic Acid (µg):
150 nM (2µg/well) 
Amount of Enhancer (µl):
4 µl 
Amount of Reagent (µl):
8 µl 
Complex Incubation on Cells (hrs):
3 h 
Analysis Performed Post-Transfection (hrs):
24 h 
Transfection Efficiency (%):
Not determined 
Knockdown Efficiency (%): 80%  
 
Any modifications to the protocol?:
 
Notes:
The cells were transfected with 150nM siRNA to c-myc 3'UTR or scrambled siRNA. The RNA final concentration of 2µg/well was achieved using tRNA as a carrier together with siRNA to c-myc 3'UTR or scrambled siRNA.  
References:
c-Myc controls proliferation versus differentiation in human pancreatic endocrine cells; Carla Demeterco et al.; The Journal of Clinical Endocrinology & Metabolism 87(7):3475-3485