Le site web n’est pas disponible en français dans son intégralité. Son contenu est international et peut aller à l’encontre des règlementations locales.

Transfection Cell Database - Detailed View


Cell Line: BSC-1

Cell Line Species/Tissue: Human / Skin epithelial-like 
Transfection Reagent: TransMessenger 
Nucleic Acid:
siRNA (dsRNA) 
Growth Medium:
MEM, Pen Strep Gln 
Percent Serum (%):
10 % 
Reporter System:
GFP 
Plasmid Purification Method:
 
Plate Format:
6-well 
Number of Cells:
5x10e5 
Percent Confluence(%):
80-99 
Amount of Nucleic Acid (µg):
2 µg 
Amount of Enhancer (µl):
4 ml 
Amount of Reagent (µl):
8 ml 
Complex Incubation on Cells (hrs):
3.5 h 
Analysis Performed Post-Transfection (hrs):
4.8 h 
Transfection Efficiency (%):
52 % 
Knockdown Efficiency (%):  
 
Any modifications to the protocol?:
 
Notes:
Tried Effectene and Electroporationin parallel - did not give as good results  
References: