| Cell Line Species/Tissue: |
Human
/
kidney epithelial |
| Transfection Reagent: |
TransMessenger |
Nucleic Acid:
|
in vitro transcribed RNA |
Growth Medium:
|
Dulbeco, Pen Strep |
Percent Serum (%):
|
10 % |
Reporter System:
|
GFP |
Plasmid Purification Method:
|
|
Plate Format:
|
6-well plate |
Number of Cells:
|
|
Percent Confluence(%):
|
80-99 % |
Amount of Nucleic Acid (µg):
|
2 µg |
Amount of Enhancer (µl):
|
4 µl |
Amount of Reagent (µl):
|
8 µl |
Complex Incubation on Cells (hrs):
|
3.5 h |
Analysis Performed Post-Transfection (hrs):
|
4.5 h |
Transfection Efficiency (%):
|
82 % |
| Knockdown Efficiency (%): |
|
| |
| Any modifications to the protocol?:
|
| |
| Notes: |
| Tried Effectene and Electroporationin parallel - did not give as good results.
RNA molecule size 800 nt.
What gene did the transfected RNA encode? eGFP
RNA purification using RNeasy Kit. |
| References: |
| |