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Transfection Cell Database - Detailed View


Cell Line: MRC

Cell Line Species/Tissue: Human / Embryonic lung fibroblast  
Transfection Reagent: TransMessenger 
Nucleic Acid:
siRNA (dsRNA) 
Growth Medium:
Dulbeco, Pen Strep 
Percent Serum (%):
10 % 
Reporter System:
GFP 
Plasmid Purification Method:
 
Plate Format:
NA 
Number of Cells:
 
Percent Confluence(%):
80-99 % 
Amount of Nucleic Acid (µg):
2 µg 
Amount of Enhancer (µl):
4 µl 
Amount of Reagent (µl):
8 µl 
Complex Incubation on Cells (hrs):
3.5 h 
Analysis Performed Post-Transfection (hrs):
4.8 h 
Transfection Efficiency (%):
78 % 
Knockdown Efficiency (%):  
 
Any modifications to the protocol?:
 
Notes:
Tried Effectene and Electroporationin parallel - did not give as good results  
References: