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Transfection Cell Database - Detailed View
| Cell Line Species/Tissue: |
Mouse
/
Muscle Myoblast, Fibroblast |
| Transfection Reagent: |
PolyFect |
Nucleic Acid:
|
DNA |
Growth Medium:
|
DMEM |
Percent Serum (%):
|
15 |
Reporter System:
|
b-gal |
Plasmid Purification Method:
|
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Plate Format:
|
100-mm dish |
Number of Cells:
|
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Percent Confluence(%):
|
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Amount of Nucleic Acid (µg):
|
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Amount of Enhancer (µl):
|
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Amount of Reagent (µl):
|
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Complex Incubation on Cells (hrs):
|
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Analysis Performed Post-Transfection (hrs):
|
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Transfection Efficiency (%):
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| Any modifications to the protocol?:
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| Notes: |
| C2C12 cells were transiently co-transfected.
Cells were incubated overnight and extracts were prepared 24 h later, normalized by assaying ß-galactosidase activity, immunoprecipitated using anti-CBP antibodies, and analyzed by Western blotting. |
| References: |
| Interaction between Acetylated MyoD and the Bromodomain of CBP and/or p300; Anna Polesskaya et al., Molecular and Cellular Biology, Aug. 2001, p. 5312-5320 |
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