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Transfection Cell Database - Detailed View


Cell Line: C2C12

Cell Line Species/Tissue: Mouse / Muscle Myoblast, Fibroblast 
Transfection Reagent: PolyFect 
Nucleic Acid:
DNA 
Growth Medium:
DMEM 
Percent Serum (%):
20 
Reporter System:
 
Plasmid Purification Method:
 
Plate Format:
6-well plate 
Number of Cells:
 
Percent Confluence(%):
 
Amount of Nucleic Acid (µg):
Amount of Enhancer (µl):
 
Amount of Reagent (µl):
 
Complex Incubation on Cells (hrs):
 
Analysis Performed Post-Transfection (hrs):
 
Transfection Efficiency (%):
 
 
Any modifications to the protocol?:
 
Notes:
After 24 h, the transfected cells were trypsinized and re-plated into growth medium containing 1mg/ml G418 on 12-well plates.  
References:
The Mammalian Target of Rapamycin Regulates C2C12 Myogenesis via a Kinase-independent Mechanism; Ebru Erbay and Jie Chen; The Journal of Biological Chemistry/Vol 276/ No. 39/ Sep 28/ pp.36079-36082, 2001