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Transfection Cell Database - Detailed View


Cell Line: K562

Cell Line Species/Tissue: Human / hematopoietic 
Transfection Reagent: SuperFect 
Nucleic Acid:
DNA 
Growth Medium:
RPMI 
Percent Serum (%):
10 
Reporter System:
GFP 
Plasmid Purification Method:
QIAGEN Plasmid Kit 
Plate Format:
 
Number of Cells:
2x10^5 
Percent Confluence(%):
 
Amount of Nucleic Acid (µg):
0.5 
Amount of Enhancer (µl):
 
Amount of Reagent (µl):
Complex Incubation on Cells (hrs):
 
Analysis Performed Post-Transfection (hrs):
 
Transfection Efficiency (%):
92% 
 
Any modifications to the protocol?:
 
Notes:
 
References:
An efficient gene transfer system for hematopoietic cell line using transient and stable vectors, Journal of Biotechnology 88 (2001) 159-165, Teixeira et al.