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Transfection Cell Database - Detailed View
| Cell Line: Mesangium cell
|
|
| Cell Line Species/Tissue: |
Rat
/
|
| Transfection Reagent: |
Effectene |
Nucleic Acid:
|
DNA |
Growth Medium:
|
|
Percent Serum (%):
|
10 |
Reporter System:
|
Luciferase |
Plasmid Purification Method:
|
QIAfilter Plasmid Kit |
Plate Format:
|
6-well plate |
Number of Cells:
|
|
Percent Confluence(%):
|
|
Amount of Nucleic Acid (µg):
|
0,4 |
Amount of Enhancer (µl):
|
3,2 |
Amount of Reagent (µl):
|
10 |
Complex Incubation on Cells (hrs):
|
12-16 |
Analysis Performed Post-Transfection (hrs):
|
|
Transfection Efficiency (%):
|
~5 |
| |
| Any modifications to the protocol?:
|
| o/n transfection |
| Notes: |
| |
| References: |
| |
|