| Cell Line Species/Tissue: |
Human
/
Hepatocarcinoma |
| Transfection Reagent: |
Effectene |
Nucleic Acid:
|
DNA |
Growth Medium:
|
DMEM/10% FCS |
Percent Serum (%):
|
10 |
Reporter System:
|
GFP |
Plasmid Purification Method:
|
|
Plate Format:
|
12-well plate |
Number of Cells:
|
|
Percent Confluence(%):
|
50-80 |
Amount of Nucleic Acid (µg):
|
3 |
Amount of Enhancer (µl):
|
24 |
Amount of Reagent (µl):
|
15 or 30 |
Complex Incubation on Cells (hrs):
|
24 |
Analysis Performed Post-Transfection (hrs):
|
24 |
Transfection Efficiency (%):
|
20-30% |
| |
| Any modifications to the protocol?:
|
| Used salmon sperm DNA (150ng) with the plasmid. Added 3x more plasmid than recommended. If salmon sperm DNA was omitted, efficiency decreased to 5%. |
| Notes: |
| |
| References: |
| |