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Transfection Cell Database - Detailed View
| Cell Line: Primary astrocytes
|
|
| Cell Line Species/Tissue: |
rat
/
brain |
| Transfection Reagent: |
Effectene |
Nucleic Acid:
|
DNA |
Growth Medium:
|
DMEM |
Percent Serum (%):
|
10 |
Reporter System:
|
CAT |
Plasmid Purification Method:
|
QIAfilter Plasmid Kit |
Plate Format:
|
6-well plate |
Number of Cells:
|
|
Percent Confluence(%):
|
80 |
Amount of Nucleic Acid (µg):
|
0.4 |
Amount of Enhancer (µl):
|
3.2 |
Amount of Reagent (µl):
|
10 |
Complex Incubation on Cells (hrs):
|
|
Analysis Performed Post-Transfection (hrs):
|
|
Transfection Efficiency (%):
|
control vector/basic vector = ca. 500/1 |
| |
| Any modifications to the protocol?:
|
| |
| Notes: |
| |
| References: |
| |
|