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Transfection Cell Database - Detailed View


Cell Line: PA317

Cell Line Species/Tissue: mouse / fibroblasts (NIH/3T3 derived) 
Transfection Reagent: SuperFect 
Nucleic Acid:
DNA 
Growth Medium:
DME 
Percent Serum (%):
Reporter System:
GFP, pGreenLantern-1 (LTI) 
Plasmid Purification Method:
QIAGEN Plasmid Kits 
Plate Format:
24-well plate 
Number of Cells:
 
Percent Confluence(%):
40-80 
Amount of Nucleic Acid (µg):
Amount of Enhancer (µl):
 
Amount of Reagent (µl):
Complex Incubation on Cells (hrs):
2-5 
Analysis Performed Post-Transfection (hrs):
24-36 
Transfection Efficiency (%):
14-28% 
 
Any modifications to the protocol?:
 
Notes:
 
References:
Albritton L.M. (1997) Efficient transfection of fibroblast and epithelial cells with SuperFect Transfection Reagent. QIAGEN News 1997 No. 2, 4