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Transfection Cell Database - Detailed View


Cell Line: PTC

Cell Line Species/Tissue: mouse / kidney tubular epthelial cells 
Transfection Reagent: Effectene 
Nucleic Acid:
DNA 
Growth Medium:
MEM 
Percent Serum (%):
10 
Reporter System:
b-gal 
Plasmid Purification Method:
QIAGEN Plasmid Kit 
Plate Format:
24-well plate 
Number of Cells:
 
Percent Confluence(%):
 
Amount of Nucleic Acid (µg):
0.1 - 0.2 
Amount of Enhancer (µl):
0.8 
Amount of Reagent (µl):
2.5 
Complex Incubation on Cells (hrs):
48 
Analysis Performed Post-Transfection (hrs):
48 
Transfection Efficiency (%):
35-45% 
 
Any modifications to the protocol?:
 
Notes:
Transfection efficiency determined by X-gal staining.  
References: