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Transfection Cell Database - Detailed View


Cell Line: U-937

Cell Line Species/Tissue: Human / histiocytic lymphoma, macrophage 
Transfection Reagent: Effectene 
Nucleic Acid:
DNA 
Growth Medium:
RPMI 
Percent Serum (%):
10 % 
Reporter System:
 
Plasmid Purification Method:
QIAGEN Plasmid Kit 
Plate Format:
no information 
Number of Cells:
no information 
Percent Confluence(%):
 
Amount of Nucleic Acid (µg):
1 µg 
Amount of Enhancer (µl):
8 µl 
Amount of Reagent (µl):
25 µl 
Complex Incubation on Cells (hrs):
30 hrs 
Analysis Performed Post-Transfection (hrs):
30 hrs 
Transfection Efficiency (%):
350,000 RLU 
 
Any modifications to the protocol?:
 
Notes:
Split cells day prior to transfection. Tested DNA:Effectene ratios of 1:25 (best) & 1:50. Also tested SuperFect and achieved 75,000 RLU.  
References: