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Transfection Cell Database - Detailed View


Cell Line: MC-9

Cell Line Species/Tissue: mouse / mast cell 
Transfection Reagent: SuperFect 
Nucleic Acid:
DNA 
Growth Medium:
RPMI, 10% FCS 
Percent Serum (%):
10 
Reporter System:
G418 
Plasmid Purification Method:
silica slurry 
Plate Format:
60-mm dish 
Number of Cells:
 
Percent Confluence(%):
 
Amount of Nucleic Acid (µg):
Amount of Enhancer (µl):
 
Amount of Reagent (µl):
20 
Complex Incubation on Cells (hrs):
48 
Analysis Performed Post-Transfection (hrs):
48 
Transfection Efficiency (%):
53 colonies 
 
Any modifications to the protocol?:
 
Notes:
Split cells the day prior to transfection. Also tested Effectene and obtained 60 colonies.  
References: