Le site web n’est pas disponible en français dans son intégralité. Son contenu est international et peut aller à l’encontre des règlementations locales.

Transfection Cell Database - Detailed View


Cell Line: L6

Cell Line Species/Tissue: rat / myoblast, skeletal muscle 
Transfection Reagent: Effectene 
Nucleic Acid:
DNA 
Growth Medium:
MEM, 10% FCS 
Percent Serum (%):
10 
Reporter System:
GFP, pEGFP 
Plasmid Purification Method:
QIAGEN Plasmid Kit 
Plate Format:
6-well plate 
Number of Cells:
 
Percent Confluence(%):
 
Amount of Nucleic Acid (µg):
0.4 
Amount of Enhancer (µl):
3.2 
Amount of Reagent (µl):
Complex Incubation on Cells (hrs):
10 
Analysis Performed Post-Transfection (hrs):
 
Transfection Efficiency (%):
20% 
 
Any modifications to the protocol?:
 
Notes:
Approximately same efficiency with SuperFect. Effectene more gentle. Electroporation gave 5%.  
References: