MaXtract High Density

Pour l’extraction sûre et pratique des acides nucléiques de solvants organiques

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MaXtract High Density (200 x 2 ml)

Cat. No. / ID:  129056

MaXtract High Density Tubes (200 x 2 ml)
294,00 $US
Connexion Pour ouvrir votre compte.
Quantity
200 x 2 ml
200 x 1.5 ml
100 x 15 ml
25 x 50 ml
Ce produit ne sera plus proposé à compter du December 31, 2024 ou jusqu’à épuisement des stocks.
Le MaXtract High Density est destiné aux applications de biologie moléculaire. Ce produit n’est pas conçu pour le diagnostic, la prévention ou le traitement des maladies.

✓ Traitement automatique des commandes en ligne 24 h/24 7 j/7

✓ Assistance technique et produits pertinente et professionnelle

✓ Commande (ou réapprovisionnement) rapide et fiable

Caractéristiques

  • Extraction plus sûre des acides nucléiques de solvants organiques
  • Réduction du transfert de contaminants
  • Récupération élevée des acides nucléiques
  • Récupération pratique des acides nucléiques

Détails produit

Le MaXtract High Density simplifie l’extraction des acides nucléiques des solvants organiques (par ex. phénol/chloroforme). Le gel MaXtract forme une barrière stable entre le solvant organique et la phase aqueuse contenant des acides nucléiques, pour une récupération facile de la phase aqueuse tout en évitant le transfert de solutions organiques, de protéines et d’autres contaminants.

Performances

Le MaXtract High Density offre une récupération 30 % supérieure aux méthodes d’extraction traditionnelles. Les protéines et autres contaminants migrent vers la phase organique et l’interphase. La barrière formée par le gel MaXtract High Density dure suffisamment pour que ces contaminants restent pris sous la barrière, empêchant le transfert lors du retrait de la phase aqueuse. La phase aqueuse peut être retirée par décantage ou pipetage, pour des récupérations des acides nucléiques jusqu’à 30 % supérieures à l’utilisation des méthodes d’extraction organiques traditionnelles.

Le MaXtract High Density est fourni dans diverses tailles de tubes, permettant l’extraction de volumes d’échantillons allant de 100 µl à 20 ml.

Séparation des phases par MaXtract High Density
Phase aqueusePhénol:chloroformeChloroforme
<0,5 M NaCl, <1 mg/ml protéinesOuiOui
≥0,5 M NaClOuiOui
≥1 mg/ml protéinesOuiOui
Isolement de l’ADN plasmidiqueOuiOui
Isolement de l’ADN génomiqueOuiOui
Isolement d’ARNOuiOui

Principe

Le gel MaXtract High Density fournit une récupération sûre et rapide des acides nucléiques des extractions organiques avec des solvants tels que le phénol/chloroforme (voir schéma «  Procédure MaXtract »).
Voir les illustrations

Procédure

Ajoutez simplement la solution d’acide nucléique et de solvant organique au tube contenant le gel MaXtract. Après le mélange et la centrifugation, le gel MaXtract High Density forme une barrière stable qui sépare les phases organique et aqueuse. La barrière capture efficacement le solvant organique dangereux et les contaminants, ce qui vous permet de décanter ou de pipeter en toute sécurité la phase aqueuse contenant les acides nucléiques dans un nouveau tube (voir figure «  Extraction sûre et facile des acides nucléiques »). Si une deuxième extraction est nécessaire, elle peut être effectuée dans le même tube si le volume maximum du tube n’est pas dépassé.

La séparation avec le gel MaXtract High Density se base sur des différences de densité entre le milieu aqueux et organique. La couche organique doit avoir une densité supérieure au gel et à la phase aqueuse, et le gel doit avoir une densité supérieure à la phase aqueuse.

Voir les illustrations

Applications

Le MaXtract High Density est très adapté à l’isolation de l’ADN plasmidique, l’ADN génomique et l’ARN. Il existe également des protocoles optimisés pour des applications spécifiques, telles que la récupération de l’ADN d’agarose à point de fusion bas. Les acides nucléiques extraits avec le gel MaXtract High Density conviennent à de nombreuses applications en aval.

Données et illustrations utiles

Ressources

Safety Data Sheets (1)
Kit Handbooks (1)
For improved recovery of nucleic acids during organic extraction procedures
Technical Information and Important Notes (1)
Quick-Start Protocols (1)
MaXtract High Density
PDF (565KB)
Certificates of Analysis (1)

FAQ

Can phenol/chloroform be placed in the MaXtract tube for extraction at a later time?

Yes. The organic phase can be placed in MaXtract Low and High Density tubes without any problem 3-4 hours before preparation of the samples. Make sure to perform the centrifugation step first, so that the gel collects at the bottom of the tube.

 

FAQ ID -1299
Can MaXtract tubes be autoclaved prior to use?

No. After autoclaving, the MaXtract gel no longer has the same properties. Since the MaXtract tubes are manufactured and filled in a fully automated process, autoclaving is not required.

 

 

FAQ ID -1305
Can DNA isolated with MaXtract tubes be used for downstream applications such as restriction digestion, labeling, blotting, PCR, and automated sequencing?

DNA purified by organic extraction can principally be used for these downstream reactions. It should be noted that phenol, like all organic solvents, alters the active centre of enzymes, thus deactivating them. An ethanol precipitation should therefore generally be carried out prior to enzyme controlled downstream reactions. Potential phenol residue is thereby effectively removed.

The use of MaXtract Low and High Density tubes has the advantage that no recontamination of the sample by organic solvents or proteins occurs.

 

FAQ ID -1303
What factors determine if denatured proteins accumulate underneath, or inside the gel of the MaXtract Low and High Density Tubes?

Generally, this is determined by the density of the denatured proteins and the gel type in the MaXtract Tubes. If proteins show a higher density than the gel, they will accumulate underneath in the organic phase. If they have the same density as the MaXtract gel, they will collect in the gel. The quantity and ratio of phenol:chloroform can also influence the behavior of the proteins.

 

FAQ ID -1312
Do you have a protocol for purification of total RNA from fatty tissues using QIAzol Lysis Reagent and MaXtract High Density?

Yes, we have the following protocols:

  • Purification of total RNA from fatty tissues using QIAzol Lysis Reagent, MaXtract High Density, and the TissueRuptor (RY29).
  • Purification of total RNA from fatty tissues using QIAzol Lysis Reagent, MaXtract High Density, and the TissueLyser (RY30).
  • Purification of total RNA from fatty tissues using the RNeasy Lipid Tissue Mini Kit and MaXtract High Density (RY31).
FAQ ID -1550
Is it possible to use MaXtract for an organic extraction with a mixture of phenol and BCP (1 bromine chlorpropane)?

Yes, this application is possible with both MaXtract Low and High Density Tubes. A sufficient quantity of BCP should be used when using MaXtract High Density, in order to achieve a stable gel phase. BCP increases the density of the organic phase in the same way as chloroform.

 

-3
Does the addition of sample and organic solution to the MaXtract Tubes require a specific pipetting sequence?

No. The functional efficiency of the MaXtract Low and High Density Tubes does not depend on the pipetting sequence.

 

FAQ ID -1307
Can frozen sample material pulverized in liquid nitrogen be directly added to MaXtract tubes?

If organic solvents are added immediately following the filling of MaXtract Low and High Density Tubes with the frozen sample, it should not have any negative effect on the functionality of the tubes.

 

FAQ ID -1301
Can the MaXtract High Density Tube be used with QIAzol to isolate RNA?
FAQ ID -1304
Which density gel type is contained in the yellow or green MaXtract Tubes?

MaXtract High Density gel is found in the yellow tubes, and MaXtract Low Density gel is contained in the green tubes.

 

FAQ ID -1315
Can several extraction steps be performed in the same MaXtract tube?

Yes, if a sufficiently large MaXtract tube has been selected for the extraction it is possible to perform multiple purification steps in this tube. Sample and organic solvent are placed in the MaXtract tube, mixed and centrifuged. Organic solvent is then added again to the aqueous phase separated by the gel, followed by mixing and centrifuging. Following the last extraction step, the sample is transferred to a new tube for storage.

For the purification of samples with high protein content, we recommend using a new MaXtract tube for each extraction step.

 

FAQ ID -1302
When isolating DNA from plant cells using CTAB, should MaXtract Low or High Density be used?

CTAB as a rule increases the density of the aqueous phase. Therefore, MaXtract High Density should be used for the organic extraction of DNA from CTAB samples.

Depending on the amount of CTAB used, the density of the sample can become too high for the MaXtract High Density Tube, leading to a gel barrier above the aqueous phase. In this case, puncture the gel with the tip of a pipette and dilute the aqueous phase with either distilled water or TE buffer. Mix again and then centrifuge. However, we recommend to repeat the separation in a new MaXtract tube.

 

FAQ ID -1308
Can a phenol-chloroform ratio different from 1:1 also be used with MaXtract Low and High Density?

A phenol-chloroform ratio of 1:1 is advantageous for the phase separation when using MaXtract Low and High Density, since the MaXtract interphase has the greatest stability at this ratio.

Methods using a phenol-chloroform ratio of as much as 6:1 are also possible with MaXtract. However, MaXtract can partly precipitate onto the bottom of the tube in this case.

If the phenol-chloroform ratio is greater than 1:1, it is necessary to use MaXtract Low Density Tubes. Note that this MaXtract type is not compatible with applications in which the aqueous phase has a high density (e.g., isolation of plasmid DNA and RNA).

 

FAQ ID -1309
If both MaXtract Low Density and High Density is suitable for an application, which type is preferable to use?

In this case, both MaXtract Low and High Density Tubes can be used with the same success. We principally recommend testing both MaXtract types.

 

 

FAQ ID -1310
Are MaXtract tubes siliconized?

No, MaXtract Low and High Density tubes are not coated in any way.

 

FAQ ID -1298