QIAcube and QIAcube Connect Accessories

The following disinfectants and detergents are recommended for cleaning the QIAcube:

• Mikrozid® Liquid (Schülke & Mayr GmbH) — ethanol-based disinfectant for spraying onto surfaces (consists of 25 g ethanol and 35 g 1-propanol per 100 g Mikrozid Liquid)
• RBS®-35 Detergent Concentrate (Pierce Chemical Co.) — anionic and nonionic surfactant based detergent for cleaning the touch screen and worktable door
• RNaseZap® (Ambion) — for complete removal of RNase contamination from glass and plastic surfaces
• 0.1 M NaOH — for cleaning the worktable and racks to remove RNase contamination (alternative to RNaseZap)
• Note: Alcohol is not recommended for cleaning of the door.

Europe 

Lysetol® AF (Gigsept® Instru AF) (Schülke & Mayr GmbH) — disinfectant for submerging worktable items (consists of 14 g cocospropylene-diamine-guanidine diacetate, 35 g phenoxypropanols, and 2.5 g benzalkonium chloride per 100 g Lysetol AF, with anticorrosion components, fragrance, and 15–30% nonionic surfactants).

USA

DECON-QUAT® 100 (Veltek Associates, Inc.) — quaternary ammonium salt based disinfectant concentrate for submerging worktable items (contains 5% alkyldimethylbenzylammonium chloride and 5% alkyldimethylethylbenzylammonium chloride).

Note: If you want to use disinfectants different from those recommended, ensure that their compositions are similar to those described above.

New software or software updates can be accessed via the QIAcube Web Portal at www.qiagen.com/MyQIAcube. To access them directly, go to User Support.

No. The QIAcube is designed for fully automated processing of up to 12 samples using QIAGEN spin-column kits. Do not use spin columns manufactured by other suppliers with the QIAcube. Damage caused by use of other types of spin columns will void the instrument warranty.

See:  http://www.qiagen.com/products/catalogpagecontrols/header/qiacube/360/default.aspx for a demonstration on how the QIAcube is used with the RNeasy Mini Kit for automated purification of RNA.

No. Slightly modified QIAGEN protocols or protocols fully customised to your specific requirements can be requested from QIAGEN. Our dedicated team of specialists has already customised over 5000 protocols.

1. Go to www.qiagen.com/MyQIAcube  and enter the required information. If our application specialists require additional information, they will contact you directly.
2. You will be notified by email when your customized protocol is ready for download.
3. Click on the link in the email to access the download page.
   
   

Dedicated QIAcube kits are the RNeasy Mini QIAcube Kit, QIAamp DNA Mini QIAcube Kit, the QIAamp DNA Blood Mini QIAcube Kit, and the QIAamp Viral RNA Mini QIAcube Kit. In addition to these kits are the QIAamp PowerFecal Pro DNA QIAcube Kit, the DNeasy PowerSoil Pro QIAcube Kit using Power technology, and the DNeasy Blood & Tissue QIAcube Kit.

The QIAcube Connect has the following weight and dimensions: 

• Weight: 73 kg (160.9 lb.)
• Width: 65 cm (25.6 in.)
• Depth: 62 cm (24.4 in.)
• Height (door closed): 58 cm (22.8 in.)
• Height (door open): 86 cm (34 in.)

Please refer to Section 6.2 "Disinfection" and Section 6.6 "Periodic maintenance" of the QIAcube Connect User Manual. 

Yes, the 2 ml Sample Tubes RB (cat. no. 990381) can be used with both TissueLyser II and TissueLyser LT.

Yes, rotor and/or buckets can be removed from the QIAcube for cleaning. For information on how to remove these parts, refer to the QIAcube User Manual.

You can stop a QIAcube protocol if there is an emergency by pressing “Cancel”. To confirm that you want to stop the protocol run, press “OK”. To cancel the 'stop protocol' command, press “Cancel”.

Note: If a protocol run is stopped, the run cannot be restarted. The samples must be processed manually. The last protocol step performed by the QIAcube will be displayed in the touch screen. For more information, refer to the QIAcube User Manual.

No. The beauty of the QIAcube Connect is that you can automate your supported manual spin-column workflow flawlessly. The manual kits contain sufficient buffers and reagents to process the number of spin columns provided with the kit.

However, in addition to the purification kit, Rotor Adapters (10 x 24), cat. no 990394, and disposable tips are required:

• Filter-Tips, 200 µL (1024), cat no. 990332,
• Filter-Tips, 1000 µL (1024), cat no. 990352,
• Filter-Tips, 1000 µL wide-bore (1024), cat no. 990452.
  
  

To find out which filter-tips are required for a specific application, refer to the corresponding protocol sheet or the instructions on the QIAcube Connect touchscreen. Even easier to use are the dedicated QIAcube kits.

No. The QIAcube is equipped with an optical sensor, which determines the tip size and the number of tips loaded onto the worktable prior to starting a run.

The QIAcube can use partially used tip racks from previous runs, provided that the racks contain sufficient tips to process the number of samples loaded for the current run.