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EZ1&2 RNA Tissue Mini Kit (48)
Cat. No. / ID: 959034
For 48 preps: Reagent Cartridges (Tissue RNA), Disposable Filter-Tips, Disposable Tip-Holders, Sample Tubes (2 ml), Elution Tubes (1.5 ml), Buffer RLT, RNase-free DNase I, RNase-free water
The EZ1&2 RNA Tissue Mini Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
✓ 24/7 automatic processing of online orders
✓ Knowledgeable and professional Product & Technical Support
✓ Fast and reliable (re)-ordering
Features
- Purification of up to 30 µg total RNA
- Reliable processing of up to 10 mg easy-to-lyse tissues
- RNase-free setup for reliable results
- Scalable elution volumes
- Easy protocol setup
Product Details
The EZ1&2 RNA Tissue Mini Kit contains all required reagents and labware for automated purification of total RNA from up to 10 mg standard tissue samples using magnetic particle technology. Reagents are supplied in prefilled and sealed reagent cartridges, which ensures speed and convenience in loading the instrument.
The EZ1&2 RNA Tissue Mini Kit is automated with the EZ2 Connect (1–24 samples per run). This kit can also be used with the EZ1 Advanced XL (1–14 samples per run).
Performance
The yields and high quality of total RNA purified using the EZ1&2 RNA Tissue Mini Kit are consistent and reproducible (see figures " Efficient purification and reproducible yields" and " Consistent highly pure RNA").
See figures
Principle
Automated purification with the EZ1&2 RNA Tissue Mini Kit requires use of one of the following:
- the EZ2 Connect
- the EZ1 Advanced XL and the EZ1 Advanced XL RNA Card
EZ1 cards are preprogrammed with protocols that guide the user through worktable setup and provide the instrument with commands for the purification procedure.
Using silica-coated magnetic-particle technology, the easy-to-use instrument allows purification of 1–24 samples (EZ2 Connect) or 1–14 samples (EZ1 Advanced XL) in a single run. Presealed reagent cartridges and the automatic sensor-controlled lock of the instrument door reduce the chances of environmental contamination or degradation due to RNases. The automated procedure provides high repeatability and linear yields over a range of starting amounts. The flexibility to purify high-quality total RNA from a wide range of tissue types ensures that samples can be purified for a range of gene expression analysis applications. RNA yield using the EZ1&2 RNA Tissue Mini Kit is dependent upon many variables, including sample weight, tissue type and developmental state.
Procedure
Tissues are first lysed and homogenized in Buffer RLT. RNA is then purified in one step on the instrument following the automated EZ1&2 RNA procedure. For certain RNA applications that are sensitive to very small amounts of DNA, an integrated DNase digestion step removes genomic DNA contamination. Total RNA is eluted in 200 µl, 100 µl, or 50 µl, ready to use in all downstream applications.
Applications
The high-quality RNA obtained using the EZ1&2 RNA Tissue Mini Kit is suited for use in many applications, such as:
- Gene expression analysis using real-time RT-PCR technologies
- Gene expression analysis using microarray technologies
- Oncology and pathology research
Supporting data and figures
Efficient purification and reproducible yields.
Using the EZ1&2 RNA Tissue Mini procedure, total RNA was purified from 10 mg samples of rat kidney and liver and from 5 mg samples of rat pancreas, spleen, and lung. RNA was purified from 3 replicate samples of each tissue type. Yields of total RNA were determined by absorbance (A260) with correction for background (A320). CV kidney = 15%; CV liver = 1%; CV pancreas = 8%; CV spleen = 10%; CV lung = 22%.
Specifications
| Features | Specifications |
|---|---|
| Applications | PCR, real-time RT-PCR, microarray |
| Time per run or per prep | 40 minutes |
| Technology | Magnetic particles |
| Elution volume | 50–200 µl |
| Processing | Automated |
| Format | Sample tube |
| Stabilization | Yes |
| For automated processing | EZ2 Connect instruments or EZ1 Advanced XL |
| Main sample type | Tissue samples |
| Sample amount | 5–10 µg |
| Yield | <30 µg |
| Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein | RNA |
Resources
Quick-Start Protocols (2)
Brochures & Guides (2)
User-Developed Protocols (1)
Supplementary Protocols (1)
Safety Data Sheets (1)
Kit Handbooks (1)
Certificates of Analysis (1)
FAQ
Is training of lab personnel included with the purchase of a BioRobot EZ1?
Are the new EZ1 RNA Kits (cat. no. 958034; 959034; 956034) compatible with the previous EZ1 RNA Card (cat. no. 9015590) or EZ1 RNA Universal Tissue Card (cat. no 9016384)?
Do you have a protocol for the isolation of total nucleic acids from animal and human tissues on the BioRobot EZ1?
How can we get our BioRobot EZ1 upgraded when new protocols are launched?
Where can I find information about the worktable setup on the BioRobot EZ1?
Do the foils sealing the EZ1 Reagent Cartridges have to be manually removed prior to loading the robot?
What has to be done to an RNA sample before loading it onto an Agilent Bioanalyzer?
Do you have a protocol for the isolation of total RNA from paraffin-embedded sections on the BioRobot EZ1?
Do you have a protocol for the isolation of DNA from fungi using the BioRobot EZ1?
How do I safely inactivate biohazardous flow-through material?
What disposables are required for nucleic acid isolations on the BioRobot EZ1?