Products
For Research Use Only. Not for use in diagnostics procedures. No claim or representation is intended to provide information for the diagnosis, prevention, or treatment of a disease.
Features
- Sensitive quantitative assay
- Specific quantification of type A, B, and D mutations
- Accurate quantification relative to ABL control gene expression
- Optimal quality control and ISO 13485 certification
- Easy workflow with ready-to-use solutions
Product Details
ipsogen NPM1 mut A, B&D MutaQuant Kits are intended for research use only and are not for use in diagnostic procedures. The kits are for real-time PCR on the Rotor-Gene Q and other real-time PCR instruments. ipsogen NPM1 mut A, B&D MutaQuant Kits provide reagents optimized for reliable and sensitive detection and quantification of specific mutations (type A, B, and D) in NPM1 gene transcripts.
Performance
To ensure highest sensitivity, ipsogen NPM1 mut A, B&D MutaQuant Kits have been optimized to detect the type A, B, and D mutations in NPM1 gene transcripts over a wide dynamic range (see figure " Sensitive detection of ABL and NPM1 mut A, B, and D standards").
See figures
Principle
ipsogen NPM1 mut A, B&D MutaQuant Kits are ready-to-use kits for the detection and quantification of type A, B, and D mutations in human RNA already identified as carrying the respective mutation in the NPM1 gene (see figure " NPM1 alleles"). Results are quantified relative to expression of the ABL control gene (see figure " ABL control gene transcript"). The kits provide high levels of sensitivity and reproducibility. Each ipsogen NPM1 mut A, B&D MutaQuant Kit provides 3 standard dilutions for the ABL gene and 5 standard dilutions for each of the NPM1 gene mutations.
See figures
Procedure
The first step is reverse transcription of total RNA in a sample into cDNA. The second step is the amplification of DNA by real-time PCR. Using ipsogen NPM1 mut A, B&D MutaQuant Kits provides reliable detection and quantification of type A, B, and D mutations in NPM1 gene transcripts. Simply start the reaction using the optimized protocols described in the kit handbook.
Applications
ipsogen NPM1 mut A, B&D MutaQuant Kits enable sensitive and reliable detection and quantification of type A, B, and D mutations in NPM1 gene transcripts for research use only.
Supporting data and figures
Sensitive detection of NPM1 mut A and ABL standards.
Detection of [A] NPM1 mut A standards (M1 to M5) with 101, 102, 103, 105, and 106 copies/5 μl and [B] ABL standards (C1, C2, C3) with 103, 104, and 105 copies/5 μl.
Resources
Kit Handbooks (2)
Safety Data Sheets (1)
Certificates of Analysis (1)