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Cat. No. / ID: 331535
✓ 24/7 automatic processing of online orders
✓ Knowledgeable and professional Product & Technical Support
✓ Fast and reliable (re)-ordering
QIAseq miRNA Library QC PCR Assays and Arrays enable rigorous quality control of both sample quality before NGS library preparation as well as NGS performance post-sequencing. Amplification of the QIAseq miRNA Library QC Spike-ins – a comprehensive set of spike-ins added during RNA isolation or to isolated RNA – allows assessment of the reproducibility and linearity of the NGS reads mapped to these exogenous sequences, ensuring optimal miRNA sequencing results.
QIAseq miRNA Library Kits are recommended for gel-free library construction miRNA, piRNA and small RNA on Illumina and Thermo Fisher Scientific NGS instruments.
QIAseq miRNA Library QC PCR Panel Kit | QIAseq miRNA Library QC Spike-Ins | |
---|---|---|
Compatible with biofluid samples | ✓ | ✓ |
Compatible with cells/tissue | — | ✓ |
Evaluate RNA yield | ✓ | — |
Evaluate miRNA/small RNA quality | ✓ | — |
Evaluate library quality | ✓ | ✓ |
Evaluate NGS reproducibility | ✓ | ✓ |
Evaluate technical outliers | ✓ | ✓ |
Addition of QIAseq miRNA Library QC Spike-Ins during RNA isolation enables monitoring of the comparability and reproducibility of the whole process from RNA isolation to NGS data analysis.
QIAseq miRNA Library QC Spike-Ins can also be added directly to RNA samples before small RNA library preparation. For more accurate ratios of spike-ins vs. endogenous miRNAs in other sample types or when using isolated RNA samples, experimental titration of QIAseq miRNA Library QC Spike-Ins is recommended.
The protocol consists of 5 steps:
1. Addition of 52 QIAseq miRNA Library QC Spike-Ins to the samples during RNA isolation
2. cDNA synthesis, including UniSp6 Spike-In
3. qPCR reactions
4. Sample evaluation and NGS of accepted samples
5. Evaluation of the QIAseq miRNA Library QC Spike-Ins data
The QIAseq miRNA Library QC Spike-In controls enable you to monitor the technical quality of the RNA isolation and cDNA synthesis and the presence of PCR inhibitors in the sample.
NGS interpretation:
The GeneGlobe data analysis center for QIAseq miRNA library kits will automatically align and report on the QIAseq miRNA spike-ins in addition to the aligned small/miRNA/piRNA from your sample.
qPCR Panel and Assay interpretation:
NGS analysis: