Buffer RLT

For lysis of cells and tissues before RNA isolation

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Buffer RLT (220 ml)

Cat. No. / ID: 79216

220 ml RNeasy Lysis Buffer

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✓ 24/7 automatic processing of online orders

✓ Knowledgeable and professional Product & Technical Support

✓ Fast and reliable (re)-ordering

Product Details

Buffer RLT is a lysis buffer for lysing cells and tissues prior to RNA isolation and simultaneous RNA/DNA/Protein isolation. When following RNeasy Plus or AllPrep DNA/RNA procedures, Buffer RLT Plus should be used.

Resources

Publications

Transcriptional regulation of uterine vascular endothelial growth factor during early gestation in a carnivore model, Mustela vison.

Lopes FL; Desmarais J; Ledoux S; Gévry NY; Lefevre P; Murphy BD;

J Biol Chem; 2006; 281 (34):24602-11 2006 Jun 21 PMID:16790435

FAQ

The exact composition of Buffer RLT is confidential. This buffer is a proprietary component of RNeasy Kits. Buffer RLT contains a high concentration of guanidine isothiocycanate, which supports the binding of RNA to the silica membrane. Buffer RLT can be purchased separately (cat. no. 79216)

Note: note that ß-mercaptoethanol should be added to Buffer RLT before use to effectively inactivate RNAses in the lysate (10 µl ß-Mercaptoethanol per 1 ml Buffer RLT).

FAQ ID -2793

Always dispose of potentially biohazardous solutions according to your institution’s waste-disposal guidelines. Although the lysis and binding buffers in QIAamp, DNeasy, and RNeasy kits contain chaotropic agents that can inactivate some biohazardous material, local regulations dictate the proper way to dispose of biohazards. DO NOT add bleach or acidic solutions directly to the sample-preparation waste. Guanidine hydrochloride in the sample-preparation waste can form highly reactive compounds when combined with bleach.
Please access our Material Safety Data Sheets (MSDS) online for detailed information on the reagents for each respective kit.

FAQ ID -12