| Cell Line Species/Tissue: |
Human
/
osteosarcoma |
| Transfection Reagent: |
HiPerFect |
Nucleic Acid:
|
siRNA (dsRNA) |
Growth Medium:
|
MEM |
Percent Serum (%):
|
10 % FCS |
Reporter System:
|
|
Plasmid Purification Method:
|
|
Plate Format:
|
24-well |
Number of Cells:
|
4x10e4 |
Percent Confluence(%):
|
80 % |
Amount of Nucleic Acid (µg):
|
5 nM |
Amount of Enhancer (µl):
|
|
Amount of Reagent (µl):
|
3 µl |
Complex Incubation on Cells (hrs):
|
48 h |
Analysis Performed Post-Transfection (hrs):
|
48 h |
Transfection Efficiency (%):
|
70 % |
| Knockdown Efficiency (%): |
20 % |
| |
| Any modifications to the protocol?:
|
| None |
| Notes: |
| |
| References: |
| |