| Cell Line Species/Tissue: |
Human
/
Neuroblastoma |
| Transfection Reagent: |
RNAiFect |
Nucleic Acid:
|
siRNA (dsRNA) |
Growth Medium:
|
DMEM |
Percent Serum (%):
|
|
Reporter System:
|
|
Plasmid Purification Method:
|
|
Plate Format:
|
2cm *2 dishes |
Number of Cells:
|
no information |
Percent Confluence(%):
|
|
Amount of Nucleic Acid (µg):
|
0.8 µg -1.2 µg |
Amount of Enhancer (µl):
|
|
Amount of Reagent (µl):
|
6 µl |
Complex Incubation on Cells (hrs):
|
|
Analysis Performed Post-Transfection (hrs):
|
no information |
Transfection Efficiency (%):
|
no information |
| Knockdown Efficiency (%): |
no information |
| |
| Any modifications to the protocol?:
|
| |
| Notes: |
| siRNA from QIAGEN: c-Rel siRNA (0.8 µg) or Bcl-XL siRNA (1.2 µg)
To transfect cortical cultures, the transfection complex was diluted in 300 µl of Neurobasal/B27 and added directly to the cells. It was replaced with fresh Neurobasal/B27 3 h later. To transfect SK-N-SH cells, the transfection complex was added in DMEM without serum and antibiotics. It was replaced with complete DMEM 3 h later. |
| References: |
| Cell Death and Differentiation (2005), 1–12
NF-jB factor c-Rel mediates neuroprotection elicited by mGlu5 receptor agonists against amyloid b-peptide
toxicity
M Pizzi*,1, I Sarnico1, F Boroni1, M Benarese1, N Steimberg2,
G Mazzoleni2, GPH Dietz3, M Ba hr3, H-C Liou4 and PF Spano
|