| Cell Line Species/Tissue: |
Human
/
Pancreatic endocrine cells |
| Transfection Reagent: |
TransMessenger |
Nucleic Acid:
|
siRNA (dsRNA) |
Growth Medium:
|
DMEM+10%FCS+5.5mM Glucose |
Percent Serum (%):
|
10%FCS |
Reporter System:
|
|
Plasmid Purification Method:
|
|
Plate Format:
|
2-well chamber glass slides |
Number of Cells:
|
|
Percent Confluence(%):
|
70-80 % |
Amount of Nucleic Acid (µg):
|
150 nM (2µg/well) |
Amount of Enhancer (µl):
|
4 µl |
Amount of Reagent (µl):
|
8 µl |
Complex Incubation on Cells (hrs):
|
3 h |
Analysis Performed Post-Transfection (hrs):
|
24 h |
Transfection Efficiency (%):
|
Not determined |
| Knockdown Efficiency (%): |
80% |
| |
| Any modifications to the protocol?:
|
| |
| Notes: |
| The cells were transfected with 150nM siRNA to c-myc 3'UTR or scrambled siRNA.
The RNA final concentration of 2µg/well was achieved using tRNA as a carrier together with siRNA to c-myc 3'UTR or scrambled siRNA. |
| References: |
| c-Myc controls proliferation versus differentiation in human pancreatic endocrine cells; Carla Demeterco et al.; The Journal of Clinical Endocrinology & Metabolism 87(7):3475-3485 |