| Cell Line Species/Tissue: |
Human
/
Breast epithelium |
| Transfection Reagent: |
SuperFect |
Nucleic Acid:
|
DNA |
Growth Medium:
|
DMEM, 10%FCS, 1%Penicillan, 1%Streptomycin |
Percent Serum (%):
|
10 |
Reporter System:
|
Luciferase |
Plasmid Purification Method:
|
Promega Plasmid Prep |
Plate Format:
|
100-mm dish |
Number of Cells:
|
1,500,000 |
Percent Confluence(%):
|
80 |
Amount of Nucleic Acid (µg):
|
5 |
Amount of Enhancer (µl):
|
|
Amount of Reagent (µl):
|
60 |
Complex Incubation on Cells (hrs):
|
4 |
Analysis Performed Post-Transfection (hrs):
|
48 |
Transfection Efficiency (%):
|
70 |
| |
| Any modifications to the protocol?:
|
| Nothing significant |
| Notes: |
| |
| References: |
| |