LiquiChip System Fluid

For use with the LiquiChip Reader

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LiquiChip System Fluid

Cat. No. / ID: 922902

LiquiChip System Fluid (10x concentrate, 5 liters), for 50 liters working solution

121,00 US$

Quantity

✓ 24/7 automatic processing of online orders

✓ Knowledgeable and professional Product & Technical Support

✓ Fast and reliable (re)-ordering

Product Details

LiquiChip System Fluid is specially formulated for use with the LiquiChip Reader. It contains a defined concentration of salt to ensure that LiquiChip beads flow freely through the measurement cuvette, and are correctly defined and measured. LiquiChip System Fluid is provided as a stabilized concentrated stock solution, with preservative. >

Resources

FAQ

Yes, the LiquiChip System that we sell at QIAGEN is a truly open system, and can be used with all available kits that are specifically designed for Multi Analyte Profiling (xMAP), whether it is a protein based kit or a nucleic acid based kit.

FAQ ID -1156

For antibody-mediated direct detection, use of 10–50 ng labeled antibody per assay point is recommended. For indirect detection, 10 ng primary antibody and 50–200 ng labeled secondary antibody per assay point deliver optimal results.

The LiquiChip Applications Handbook contains protocols for immunoassay and subsequent detection using both direct and indirect methods.

FAQ ID -1159

It is possible to work in daylight when pipetting and handling the reagents of the LiquiChip System. However, please protect stored LiquiChip beads from light by wrapping the reaction vessels in aluminum foil. Avoid working in direct sunlight. All incubations with the beads should be done in the dark.

FAQ ID -735

The detection of the green reporter signal is triggered by the detection of the red classification fluorescence. Therefore, a specific sample value is only recorded if a red bead classification signal and a green reporter signal are measured simultaneously.

In addition, the assay mixture is covered by the LiquiChip System Fluid for the measurement in the capillary, so that only a small amount of assay mixture is around the beads during measurements. Further information can be found online on the product page for the LiquiChip System.

FAQ ID -732

No. LiquiChip beads are intended for use on LiquiChip and other xMAP instruments only. Conventional flow cytometers are not capable to discriminate LiquiChip bead codes.

FAQ ID -733

A homogeneous LiquiChip Assay is an xMAP assay with no requirement to separate free fluorophores from bead-bound fluorophores.

Since reporter fluorescence is only registered when the measured bead can be recognized as belonging to a defined bead set and as having a defined size, reporter fluorescence is derived from a bead only, and not from unbound fluorophores free in solution. Thus, there is no need to perform wash steps.

FAQ ID -476

Using the LiquiChip System, thousands of beads can be interrogated in seconds, allowing a 96-well microplate to be measured in 35–60 minutes, depending on assay set up (bead concentration per assay, total assay volume). Approximately 20-60 seconds are needed per well (including wash procedure). Using a 100-plex assay, up to 9600 data points can be measured in 1 hour.

FAQ ID -1162

Only one reporter dye can be used, because only a single laser (green reporter laser) is available to read the reporter signal using the LiquiChip Workstation. However, since multiplexing is not done via the reporter dye, but instead by using different color-coded beads identified by a red classification laser, multiplexing on this instrument is possible. QIAGEN supplies Kits performing 10-plexes for the LiquiChip System. In theory, a 100-plex is possible using this system.

FAQ ID -738

This is difficult to determine as the sensitivity highly depends on assay set-up, quality of the interactions, or antibodies used. In general, we can detect in the low pg/ml range using high affinity antibodies.

An example for the high assay sensitivity can be found on the LiquiChip System page.

FAQ ID -731