Investigator IDplex Plus Kit

Para la amplificación multiplexada de 13 locus básicos del CODIS más D2S1338, D19S433 y amelogenina, también directamente de hisopos y papeles

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Investigator IDplex Plus Kit (100)

Cat. No. / ID: 381625

Mezcla de cebador y Fast Reaction Mix que incluyen HotStarTaq Plus DNA Polymerase, ADN de control, escalera alélica IDplex Plus, estándar de tamaño de ADN 550 (BTO) y agua libre de nucleasas

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KitAccessories

Investigator IDplex Plus

Matrix Standard

Reactions

100

400

Este producto contiene sustancias reguladas por el REACH (CE 1907/2006 Anexo XIV). El uso de este producto en la UE está permitido previa exención (artículo 56 [3]). Para más información, consulte la notificación del REACH y la hoja de datos de seguridad de este producto, que encontrará en la sección “Recursos” de esta página.

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Los Investigator IDplex Kits están concebidos para aplicaciones de biología molecular en análisis forenses, de identificación humana y en pruebas de paternidad. Estos productos no están concebidos para el diagnóstico, la prevención ni el tratamiento de enfermedades. Estos productos no están disponibles en todos los países.

✓ 24/7 automatic processing of online orders

✓ Knowledgeable and professional Product & Technical Support

✓ Fast and reliable (re)-ordering

Features

Amplificación simultánea de 16 locus, incluido el núcleo CODISMuy adecuado para casos forenses o muestras de referenciaProtocolos sencillos con Fast Reaction Mix lista para usarDiseño optimizado del cebador para reducir el solapamiento de marcadores

Product Details

Investigator IDplex Plus Kit es un ensayo STR multiplexado para la identificación humana que comprende los 13 locus básicos del CODIS (Combined DNA Index System), D2S1338, D19S433 y amelogenina.Los kits utilizan nuestra tecnología de ciclado rápido, que permite la amplificación en unos 90 minutos. Altamente sensible y con resultados rápidos y fiables a partir de trazas de ADN, el Investigator IDplex Plus Kit es adecuado para todas las aplicaciones forenses y pruebas de paternidad. El analizador genético se calibra con BT5 Matrix.Investigator IDplex Kit cumple los requisitos de la norma ISO 18385.

Performance

El Investigator IDplex Kit utiliza tecnología de ciclado rápido para proporcionar resultados fiables en ensayos de STR en unos 90 minutos a partir de ADN extraído.La Fast Reaction Mix se basa en la tecnología de PCR multiplexada de QIAGEN de eficacia probada que se ha optimizado para aumentar la tolerancia a los inhibidores. El rendimiento del ensayo probado en presencia de concentraciones crecientes de 6 tipos de inhibidores (ácido húmico, hematina, ácido tánico, carmín de índigo, calcio y colágeno) muestra una alta sensibilidad y una resistencia superior a los inhibidores. Esto permite obtener resultados fiables y de alta calidad a partir de muestras de casos.El Investigator IDplex Kit se ha validado y evaluado mediante el termociclador GeneAmp 9700 y el Applied Biosystems 3500 Genetic Analyzer.La cantidad recomendada de ADN en condiciones normales para utilizar con el Investigator IDplex Plus Kit es de 0,5 ng. Las validaciones internas mostraron resultados estables y equilibrados con entre 0,2 y 2 ng de ADN y resultados fiables con cantidades inferiores a 0,1 ng de ADN.

Principle

El Investigator IDplex Kit utiliza tecnología PCR de ciclado rápido de QIAGEN para la amplificación rápida y simultánea de los 13 locus básicos del CODIS (Combined DNA Index System), D2S1338, D19S433 y amelogenina. Los cebadores cuidadosamente diseñados reducen el solapamiento de los alelos de los locus, minimizando así la probabilidad de interpretaciones erróneas.

Procedure

Los 15 marcadores STR autosómicos polimórficos (D2S1338, D3S1358, D5S818, D7S820, D8S1179, D13S317, D16S539, D18S51, D19S433, D21S11, CSF1PO, FGA, TH01, TPOX y vWA) y la amelogenina específica del sexo se amplifican simultáneamente en una única PCR y se etiquetan con uno de los colorantes enumerados en la tabla «Colorantes y marcadores correspondientes del Investigator IDplex Plus Kit». Colorantes y marcadores correspondientes del Investigator IDplex Plus KitColoranteMarcador6-FAMAmelogeninaTH01D3S1358vWAD21S11BTGTPOXD7S820D19S433D5S818D2S1338BTYD16S539CSF1POD13S317FGA BTRD18S51D8S1179 Los locus amplificados se someten a electroforesis capilar. Los resultados pueden analizarse con programas informáticos específicos, como GeneMapper ID-X. Consulte la tabla «Especificaciones técnicas» para más información.

Applications

Los Investigator IDplex Plus Kits sirven para la generación rápida y segura de perfiles de ADN a partir de sangre, frotis bucales y tinciones forenses o muestras de referencia y son muy adecuados para:Bases de datos criminales, referencias forenses o muestras de casosPruebas de paternidadEstudios de genética poblacionalOtras aplicaciones de pruebas de identificación humana

Especificaciones técnicasCaracterísticasInvestigator IDplex Plus KitVolumen de entrada de ADNHasta 15 µlVolumen por reacción25 µlMatrizBT5Colorantes fluorescentes6-FAM, BTG, BTY, BTOAnalizadores genéticosABI PRISM 3100/3100 Avant, Applied Biosystems 3130/3130xl o Applied Biosystems 3500 Genetic AnalyzersSoftware para análisisApplied Biosystems GeneMapper ID o GeneMapper ID-X

Resources

Advanced sample collection, automation and STR solutions for kinship testing

For further information on which version of the v11 template files is suitable for your needs, please refer to the Template File Info Note.

The most comprehensive and versatile coverage of current international STR standards for capillary electrophoresis analysis

Quality initiatives for human identity testing and forensics

FAQ

Yes. The Fast Reaction Mix of the Investigator IDplex Plus Kit contains HotStarTaq Plus enzyme, Mg2+, and dNTPs in a pre-optimized concentration. Only Investigator IDplex Plus Primer Mix and template DNA need to be added. Nuclease-free Water should be added to complete the reaction volume.

FAQ ID -3008

We recommend first adding master mix to the PCR plate and then adding the punch to avoid punches getting lost or mixed up due to electrostatic effects. Furthermore, we strongly recommend spinning down the PCR plate after setup to ensure all punches get fully submerged.

FAQ ID -3096

Yes. The protocol is generic and has successfully been tested on various commonly used instruments.

FAQ ID -3089

Yes. The protocol of the Investigator IDplex Plus Kit is generic and has been successfully tested on various commonly used instruments.

FAQ ID -3009

15 µl input volume can be used with the Investigator IDplex Plus Kit.

FAQ ID -3014

Amplification of Control DNA 9948 is appropriate as a PCR performance check. However, if you have access to reference samples at constant quality (for example, swabs or FTA donated by lab personnel), these samples may be used as additional in process controls.

FAQ ID -3094

If you are using an ABI PRISM 3100-Avant/3100 or 3130/3130xl Genetic Analyzer, please use Any5Dye, if available. If you are using the Applied Biosystems 3500/3500xL Genetic Analyzer, please use filter set AnyDye. Otherwise, filter set G5 should be used. If you are using an ABI PRISM 310 Genetic Analyzer, filter set G5 should be used.

FAQ ID -3017

No. Unlike some other QIAGEN STR products (e.g., the Investigator Argus Y-12 QS Kit) there is no QS quality sensor included in the Investigator IDplex Plus Kit.

FAQ ID -3011

No. Up to 2 µl can be added on top of a 25 µl reaction.

FAQ ID -3088

We do not recommend using more than one punch of 1.2 mm diameter for blood or buccal cells on FTA, or 2 µl of a crude buccal swab lysate. To adapt protocols to the type and quality of samples used in your laboratory, and to the CE instrumentation in use, adjust PCR cycle numbers and CE injection conditions.

FAQ ID -3090

Yes. Use of indicator cards helps to ensure sample is taken from an appropriate area of the FTA card. This may significantly improve success rates compared to procedures using non-indicator cards.

FAQ ID -3095

The optimum sample DNA input range of the Investigator IDplex Plus Kit is between 200–500 pg. However, reliable results can be obtained with less than 100 pg. For higher amounts of input DNA, consider adjusting the PCR cycle number.

FAQ ID -3015

No. Amplification products of marker FGA have a slightly different size between IDplex Plus and IDplex GO! kits due to a change in the fluorescent dye linker used. Use of the IDplex Plus ladder will frequently cause off ladder calls for FGA alleles.

FAQ ID -3093

Yes. The Fast Reaction Mix contains Taq enzyme, Mg²⁺, and dNTPs in a pre-optimized concentration. Only IDplex GO! Primer Mix and sample needs to be added.

FAQ ID -3087

Yes. Sample materials used for direct amplification vary with regard to quality and quantity of DNA present, depending on sampling materials, procedures, and storage conditions. In order to obtain the highest possible success rates in routine use, it is essential to run a representative batch of samples for evaluation of optimal conditions. Furthermore, we recommend revisiting this aspect after a certain period of routine use to ensure the evaluated conditions are optimal and re-adjust if necessary.

FAQ ID -3091

No, the primer site locations of the markers in the Investigator IDplex Plus Kit have not been changed. Only minor changes have been introduced for some markers, e.g., to improve A addition to the PCR products.

FAQ ID -3012

Matrix BT5 should be used with Investigator IDplex Plus Kit.

FAQ ID -3016

The BT5 matrix standard is designed for use with our 5-dye Investigator STR Assays. We do not disclose the containing dyes.

FAQ ID - 3364

Yes. For example, cycle numbers can be increased to 32 or 34 in order to obtain higher signals for very diluted samples. However, please note that this promotes the amplification of nonspecific products or amplification from spurious background DNA.

FAQ ID -3010

The Investigator IDplex Plus Kit contains Primer mix, Fast Reaction Mix, Control DNA allelic ladder IDplex Plus, DNA size standard 550 (BTO), and nuclease-free water.

FAQ ID -3013

The template files for the Investigator IDplex Plus Kit for various softwares and analyzers can be downloaded from the Resource tab on the product webpage.

FAQ ID -3019

No, primer site locations of markers have not been changed. Two additional SNP primers have been added to allow balanced amplification of vWA and D16S539 alleles carrying known primer binding site mutations. The mutations covered were observed in concordance studies in samples of African origin at an elevated level. The labelled FGA forward primer has been slightly changed with regard to the linker of the fluorescent dye. The change does not affect PCR amplification, but causes a slight size shift during capillary electrophoresis. The allelic ladder therefore has been changed accordingly.

FAQ ID -3092

For Any5Dye filter set with the Investigator IDplex Plus Kit, please use:

Condition Bound: Lower 1.0 – Upper 20
Sensitivity: 0.1
Quality Value: 0.80

FAQ ID -3018